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Agarose Based Protein A Affinity

MabPurix A65

Product Number: 270765990

MabPurix A65 affinity chromatography resin is based on highly cross-linked agarose microspheres which possess inherent high hydrophilicity. Its surface is uniquely chemically modified and bonded with an alkali-resistant recombinant Protein A ligand that is specifically designed for the separation and purification of monoclonal antibodies and recombinant protein biomolecules containing Fc fragments.

Product Characteristics

  • High binding capacity
  • High hydrophilicity
  • Good alkali resistance and long resin lifetime
  • Polymer matrix, low back pressure, fast flow rate
  • High batch-to-batch reproducibility, easy to scale up

Select Volume

SKU: 270765990-5

$50.00

Specifications

PropertyValue
MatrixHighly cross-linked agarose microspheres
LigandAlkaline resistant recombinant protein A
Ligand Covalent BondingThioether bond
Average Particle Size*~65 μm
Dynamic Binding Capacity*≥ 60 mg Human IgG/mL resin (6 min residence time)
Operating Pressure≤0.3 MPa (3 bar)
Flow Rate*550 cm/hr (operating pressure 2 bar)
pH Stability (operation)3-12
pH Stability (CIP)2-13.7
CIP0.1-0.5 M NaOH
Operating Temperature10-30 °C, avoid freezing
Mobile Phase Compatibility*All commonly used buffers such as phosphoric acid and acetic acid. Other reagents: 8.0M urea, 6.0M guanidine hydrochloride, 20% ethanol, acetonitrile, 1.0M acetic acid, 5% isopropanol, etc
Storage Conditions2-8 °C, 20% ethanol or 2% benzyl alcohol
Transportation Requirements2-8 °C, 20% ethanol or 2% benzyl alcohol
Typical ApplicationsAntibodies, Fc fusion proteins, ADC

* Test conditions may vary. Please refer to the User Manual for detailed testing protocols.

Ordering Information

VolumeSKUPriceAction
1 mL270765990-1Request Sample
5 mL270765990-5$50.00
25 mL270765990-25Request Quote
100 mL270765990-100Request Quote
500 mL270765990-500Request Quote
1 L270765990-1LRequest Quote
5 L270765990-5LRequest Quote

Documents

User Manual

PDF · 1.2 MB

Technical Data Sheet

PDF · 850 KB

Certificate of Analysis (CoA)

PDF · 120 KB

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