mRNA Purification
mRNA Purification Solutions
Complete workflow from IVT reaction cleanup to LNP-grade mRNA. Our dT affinity resins provide highly specific capture of poly-A tailed mRNA, while HIC/RPC polishing removes critical dsRNA impurities.
mRNA Purification Process
dT Affinity-based capture workflow
1
1. Capture
dT Affinity
~85%
Purity
2
2. Polishing
HIC or RPC
>95%
Purity
3
3. Formulation
UF/DF
LNP Ready
Purity
mRNA Purification Workflow
dT Affinity Capture Strategy
Monomix dT20
Proteomix POR50-dT20
HIC Resins
RPC Resins
dT
Affinity
Capture
Buffer
Exchange
HIC
/
RPC
Polish
Ultrafiltration
Formulation
Captures via
Poly-A tail
Removes
dsRNA,
DNA template,
enzymes
Key Purification Challenges
Critical impurities addressed by our mRNA purification platform.
dsRNA Removal
Double-stranded RNA byproducts trigger immunogenic responses. Our HIC/RPC polishing effectively separates dsRNA from mRNA.
DNA Template
Residual plasmid DNA template must be removed to <10 ng/mg. dT affinity naturally excludes non-polyadenylated DNA.
Enzyme Clearance
T7 polymerase and capping enzymes are efficiently removed through our multi-step purification workflow.
Recommended Products
Key resins for mRNA purification workflows.